anchored PCR


anchored PCR
(= anchored polymerase chain reaction)
Variety of polymerase chain reaction in which only enough information is known to make a single primer. A known sequence is thus added to the end of the DNA, perhaps by enzymic addition of a polynucleotide stretch or by ligation of a known piece of DNA. The PCR can then be performed with the gene-specific primer and the anchor primer.

Dictionary of molecular biology. 2004.